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fitc conjugated anti sheep cd45 antibody  (Bio-Rad)


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    Structured Review

    Bio-Rad fitc conjugated anti sheep cd45 antibody
    Fitc Conjugated Anti Sheep Cd45 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+sheep+cd45+conjugated+to+fitc/Mouse+anti+Sheep+CD45/pmc12966326-219-32-37
    Average 94 stars, based on 90 article reviews
    fitc conjugated anti sheep cd45 antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Staining:

    Article Title: Punch-excised explants of bovine mammary gland to model early immune response to infection.
    Article Snippet: Tissue digestions were filtered through a 100-μm cell strainer (352360, Falcon, Durham, USA), centrifuged at 400 × g for 10 min and cell pellets were washed twice with FACS buffer (DPBS without Ca2+ and Mg2+ supplemented with 2% (v/v) normal goat serum (16210– 072, Gibco, Auckland, New Zealand) and 2 mmol/L EDTA. .. For surface markers staining, cells were processed as described by Cunha et al. [30] using the following antibodies: Anti-bovine G1 (WSC0608B, clone CH138A, KingFisher, Saint Paul, USA), anti-sheep CD45 conjugated to FiTC (MCA832F, clone CC1, BioRad, USA) and anti-human CD14 conjugated to Alexa 647 (MCA1568A647, clone TUK4, BioRad, USA). .. Anti-mouse IgM conjugated to PE-Cy7 (406513, clone RMM-1, Biolegend, San Diego, USA) was used as secondary antibody.

    Article Title: Punch-excised explants of bovine mammary gland to model early immune response to infection
    Article Snippet: Alternative methods for tissue digestion were tested and are described in the Supplementary Fig. S and S . Tissue digestions were filtered through a 100-μm cell strainer (352360, Falcon, Durham, USA), centrifuged at 400 × g for 10 min and cell pellets were washed twice with FACS buffer (DPBS without Ca 2 + and Mg 2 + supplemented with 2% (v/v) normal goat serum (16210–072, Gibco, Auckland, New Zealand) and 2 mmol/L EDTA. .. For surface markers staining, cells were processed as described by Cunha et al. [ ] using the following antibodies: Anti-bovine G1 (WSC0608B, clone CH138A, KingFisher, Saint Paul, USA), anti-sheep CD45 conjugated to FiTC (MCA832F, clone CC1, BioRad, USA) and anti-human CD14 conjugated to Alexa 647 (MCA1568A647, clone TUK4, BioRad, USA). .. Anti-mouse IgM conjugated to PE-Cy7 (406513, clone RMM-1, Biolegend, San Diego, USA) was used as secondary antibody.



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